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Chapter 27: Environmental Microbiology

Be able to:

  1. Define metabolic diversity and explain the benefits of having this ability
  2. List and describe/diagram the 4 biogeochemical cycles
  3. Define biofilms and discuss its benefits to bacteria and problems for us
  4. List and describe the various zones of a pond in terms of oxygen availability and light penetration and the organisms that live in each zone

 

 

Ch. 27 Environmental Microbiology

 

Microbial Ecology:

§   relationship of microbes with each other and their environment

§   huge effect on soil, water, our food

 

§   Major ecosystems

§   Oceans, rivers, lakes, deserts, marshes, grasslands, forests and tundra

3 main levels of nutrient acquisition exist in ecosystems

      Primary producers:

§  Autotrophs - Convert CO2 to organic materials

§  Photoautotrophs - use sunlight for energy

§  Plants
§  Algae
§  Cyanobacteria
§  Anoxygenic phototrophs

§  Chemoautotrophs

§  Oxidize inorganic compounds for energy

§  serve as food source for consumers and decomposers

 

3 main levels of nutrient acquisition exist in ecosystems

       Consumers:

§   heterotrophs

§   use organic material created by producers

§   Herbivores eat primary producers

§   primary consumers

§   Carnivores consume herbivores

§   Secondary consumers

§   Carnivores that eat other carnivores

§   tertiary consumers

 

 

3 main levels of nutrient acquisition exist in ecosystems

       Decomposers:

§   heterotrophs

§   digest leftovers of primary producers and consumers

§   detritus ( fresh or partially decomposed organic matter)

§   bacteria and fungi - key players in decomposition

§   mineralization

§   complete breakdown of organic molecules to inorganic molecules

 

Metabolic Diversity

§  Few places within organisms that are sterile

§  No place in the world that is sterile

§  Microbes live in a variety of habitats due to their abilities:

§ To use a variety of carbon & energy sources

§ To grow under different physical conditions

§  Extremophiles live in extreme

§ pH

§ Temperature

§ Salinity

 

Microbial Habitat

§  Aquatic

§ Marine

§ Freshwater

 

§  Terrestrial

§ Air

§ soil

§ composition of microbes dependent on soil conditions

 

§  Associations with insects plants and animals

 

Associations with Animals

§  normal bacterial flora of humans and other animals

Bioluminescent bacteria:

§  associate with animals for food and protection

§  Fish: Flashlight fish and luminescent bacteria

Associations with Insects

§  Ants - live in associations with fungi

§  Aphids - live in associations with bacteria

Associations with Plants

Bacteria association with plants

§  Agrobacterium

§ produce plant tumors to gain nutrient

§  Rhizobium - mutually beneficial relationship with plants (nitrogen fixation)

Biogeochemical Cycles

§  Recycling (oxidation and reduction) of chemical elements by microorganisms

Carbon Cycle

 

The Nitrogen Cycle

 

Nitrogen fixers

§  fix nitrogen

§ most common is Rhizobium

§ Gram-negative rods

§ Infect roots of legumes ŕ root nodules.

§  Nodules where nitrogen fixation takes place.

 

The Sulfur Cycle

 

The Phosphorous Cycle

§  Phosphorous exist primarily as phosphate ions (PO43-)

§  cycle involves changes from:

§ soluble to insoluble forms

§ organic to inorganic forms based on pH

§  no volatile form to be loss to the atmosphere

 

Degradation of Synthetic Chemicals

§  Natural organic matter is easily degraded by microbes

§  Xenobiotics are resistant to degradation

 

Decomposition by Microbes

§  Bioremediation

§ Use of microbes to detoxify or degrade pollutants

§ Bioaugmentation

§ Addition of specific microbes to degrade pollutant

 

§  Composting

§ Arranging organic waste to promote microbial degradation

 

Controlling environmental pollutants

§  current methods are incineration or storage in land fills, which result in

§ more pollution

§ health risks

 

§  bioremediation

§ inexpensive

§ publicly accepted

§ non-polluting (ideally)

§ in situ treatment (at the site)

 

Biofilms

Biofilms: polysaccharide encased microbe community

§    Beneficial for bacteria:

§   adherence to surfaces

§   share nutrients

§   protection from harmful factors

§    Problems for us:

§   growth in hospital water reservoirs

§   serious consequences in hospitalized patients

Freshwater Zonation

 

Water Quality

§  Microbes

§ present in water

§ can be filtered from groundwater

§  drinking and recreational water

§ transmit pathogens to human

 

Coliforms

§  Aerobic or facultatively anaerobic, gram-negative, non–endospore forming rods that ferment lactose to acid + gas within 48 hr, at 35°C

§  Indicator organisms

§ Used to detect fecal contamination

 

Wastewater Treatment

§  sewage composed of all materials of household plumbing system

§  Effective sewage treatment:

§ Eliminate potential pathogens and toxic materials

§ Decreases organic content in sewage

 

Municipal Sewage Treatment